RESULTS FcR元 is identified as candidate human sperm-egg fusion epitope and coevolved with IZUMO1 We sought to identify new human sperm-egg binding/fusion epitopes by using a random one-bead one-compound (OBOC) combinatorial peptide library ( 10, 11) to represent synthetic egg mimics. This species specificity and the observation that Juno is lost early in gamete contact before completion of membrane fusion indicate that additional membrane recognition mechanisms are likely to be involved. Juno/Izumo1 interaction is conserved in mammals including marsupials ( 8) however, gamete fusion displays a species-specific interaction that is particularly stringent for human fertilization ( 9). Binding of these receptors is facilitated by a tetraspanin network (CD9 and CD81) in the egg membrane ( 1), such that deletion of either receptor or the tetraspanins substantially inhibits gamete fusion despite an apparently normal sperm-egg attachment ( 5– 7). Molecular recognition between the plasma membranes in the mouse involves the egg receptor on the sperm, Izumo1, a member of the immunoglobulin (Ig) superfamily, and the sperm receptor on the egg, Juno, a glycosylphosphatidylinositol-anchored nonfunctional folate receptor family member ( 2– 4). ![]() Lam, Harry Moore, and Katerina Komrskova Show FewerĪ key event of vertebrate fertilization is the membrane fusion of the gametes, enabling the spermatozoon to enter the ooplasm and trigger the resumption of meiosis ( 1). Jana Vondrakova, Michaela Frolikova, Lukas Ded, Jiri Cerny, Pavla Postlerova, Veronika Palenikova, Ondrej Simonik, … Show All …, Zuzana Nahacka, Krystof Basus, Eliska Valaskova, Radek Machan, Allan Pacey, Zuzana Holubcova, Pavel Koubek, Zuzana Ezrova, Soojin Park, Ruiwu Liu, Raghavendran Partha, Nathan Clark, Jiri Neuzil, Masahito Ikawa, Kent Erickson, Kit S.
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